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GRK 1482 Jahrbuch 2011-2014

JUNIOR PRINCIPAL INVESTIGATORS GRK Annual Report 2011-2014 | Page 29 Education / Degrees 1979 Diploma in Biology, Technische Universität München 1979 - 1981 Max-Planck Institute for Biochemistry, Martinsried, Germany 1981 Ph.D., Technische Universität München Positions held 1981 - 1983 Postdoctoral fellow, Department of Microbiology, Technische Universität München 1983 to date Group leader, Department of Microbiology, Technische Universität München Research Interests and Achievements The major interests of the group concern global phylogeny and prokary- otic systematics, the development and application of nucleic acid based detection and identification systems, probe mediated taxon specific cell enrichment as well as development of supporting software tools. Since three decades comprehensive phylogenetic analyses are based on comparative sequence analyses of conserved markers such as rRNAs, translation elongation- and initiation factors, ATPases, heat shock pro- teins, gyrases, recombinases and RNA polymerases. The group contri- butes to Bergey’s Manual of Systematic Bacteriology and the respective taxonomic outlines (http://www.bergeys.org/outlines.html), the ‚All Species Living Tree Project‘ providing processed rRNA sequences and phylogenetic trees for all prokaryotic type taxa (http://www.arb-silva.de/projects/living- tree/), as well as one of the leading rRNA data repositories: the SILVA rRNA sequence database project (http://www.arb-silva.de). The world wide used ARB software package for sequence analyses and database management is developed and maintained since two decades (http://arb-home.de). Probe and PCR based detection and identification systems as well as polynucleotide mediated cell enrichment techniques are currently optimized and adapted for enterococci and fecal samples. Dr. Wolfgang Ludwig Technische Universität München Microbiology Emil-Ramann-Str. 4, D-85350 Freising-Weihenstephan www.wzw.tum.de/mikrobiologie Selected Publications Ludwig, W et al. ARB: a software environment for sequence data. Nucleic Acids Res. 2004, 32: 1363-1371. Ludwig, W. Molecular Phylogeny of Microorga- nisms: Is rRNA Still a Useful Marker? In: (A. Oren and R.T. Papke, eds.) Molecular Phylogeny of Microorganisms. Caister Academic Press., 2010. ISBN 978-1-904455-67-7. Pruesse, E. Quast, C, Knittel, K, Fuchs, BM, Ludwig, W, et al. SILVA: a comprehensive online resource for quality checked and aligned riboso- mal RNA sequence data compatible with ARB. Nucleic Acids. 2007, 35:7188-7196. Yarza, P, Richter, M, Peplies, J, Euzeby, J, Amann, R, Schleifer, KH, Ludwig, W et al.The All-Species Living Tree project: A 16S rRNA-based phyloge- netic tree of all sequenced type strains. Syst. Appl. Microbiol. 2008, 31:241-250. Zwirglmaier, K, Ludwig, W, and Schleifer, KH. Im- proved Method for Polynucleotide Probe-Based Cell Sorting, Using DNA-Coated Microplates. Appl. Envirion. Microbiol. 2004, 70:494-497. Research Goals Development, optimization and evaluation of a genus specific polynucleotide based cell enrichment technique for enterococci Adaptation of the method for cell enrich- ment of the enterococci sub population from fecal samples Metagenomic studies of the enriched sub population

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